Antibacterial activity of Trigonella Foenum- groecum essential oil against skin infection with Staphylococcus aureus: In vitro and in vivo studies

  • Shurooq rayyis kadhim Department of Laboratory of clinical science, College of Pharmacy, Al-Mustansyria University,Baghdad-Iraq
  • Ahmed Rahma Abu-Raghif Department of Pharmacology, College of Medicine, Al- Nahrain University, Baghdad, Iraq
  • brahim Salih Abbas Departments of Pharmacognacy, College of Pharmacy, Al-Mustansirya University,Baghdad-Iraq
  • Maysaa ali abdul khaleq Departments of Dermatology, Al- Yarmook Teaching Hospital, Baghdad, Iraq
  • Kawther Abdul Mahdi Naji Departments of Dermatology, Al- Yarmook Teaching Hospital, Baghdad, Iraq
  • Ahmed Hamza AJMII The State company for Marketing Drugs and Medical Appliances / Ministry of Health, Baghdad, Iraq
Keywords: Antiracial activity, T. Foenum- groecum, Staphylococcus aureus

Abstract

Background: Multi- drug resistant (MDR) Staphylococcus aureus infections have become a major public health concern in both hospital and community settings.

Objective: to investigate the antibacterial activity of T.Foenum- groecum essential oil against skin infection with S. aureus and to study probable synergistic activity in combination with Clindamycin.

Type of the study: Cross-sectional study.

Methods:Antibacterial activity of T. Foenum- groecum essential oil extract (1.2gm/100 μl) was investigated in multi- drug resistance (MDR) Staphylococcus aureus specimen isolated from patients with skin infection in Baghdad. T. Foenum- groecum use externally for cellulites and skin inflammation due to the presence of diosgenin .fast liquid chromatography was used to separate these components.

Results: Antibiotics combinations revealed that T. Foenum- groecum essential oil with Clindamycin against MDR isolates of S. aureus showed a synergistic effect when used as 1/4 MIC for each antimicrobial. In vivo study was executed to determine antibacterial activity of these compounds by induction of skin infection with Staphylococcus aureus in mice and the treatment begun after 4hrs later and continue to seven days then skin biopsy was taken and sent for histopathological examination.

Conclusions: According to the results of this study, we can conclude that T. Foenum- groecum essential oil has remarkable antistaphylococcal activity. Combination of T. Foenum-groecum essential oil with Clindamycin was more effective than Clindamycin alone in treatment of skin infection with Staphylococcus aureus.

Published
2016-01-05